Institut de Chimie Moléculaire et des Matériaux d'Orsay

Laboratoire de Chimie Bioorganique et Bioinorganique - LCBB

Accueil LCBB > Thématiques > Thème 2 > Thème 2.7

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Thème 2 (J.-P. Mahy)
Conception et synthèse de nouveaux complexes métalliques
et étude de leurs interactions avec les biomolécules

R. Ricoux, F. Avenier, W. Ghattas, J.-P. Mahy

 

2.7. Elaboration de Métalloenzymes artificielles (Artzymes) pour des applications en catalyse énantiosélective
R. Ricoux, W. Ghattas

 

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αRep is a new family of artificial proteins based on a thermostable alpha-helical repeated motif. One of its members, αRep A3, forms a stable homo-dimer with a wide cleft that is able to receive metal complexes and thus appears as suitable for generating new artificial biocatalysts.

A phenanthroline ligand was covalently attached to the unique cysteine of each F119C and Y26C αRep A3 variant and the corresponding biohybrids were found to be able, in the presence of Copper(II), to catalyze enantioselectively the Diels-Alder (D-A) cycloaddition with up to 62% ee.

This study validates the choice of the αRep A3 dimer as a protein scaffold and provides a new promising route for the design and production of new enantioselective biohybrids based on entirely artificial proteins issued from a highly diverse library.

Chem. Eur. J. (2017) 23, 10156-10166